《植物生理学报》 2009, 45(11): 1081-1084

豌豆下胚轴细胞的超微结构对低温的适应性变化

陈旭微*

温州大学教师教育学院, 浙江温州325035

通信作者：陈旭微；E-mail: chenxuwei@126.com；Tel: 0577-86680941

摘　要：

采用透射电子显微镜观察经10 ℃低温处理2 d的豌豆下胚轴细胞超微结构的结果显示, 10 ℃低温处理后, 豌豆下胚轴 细胞超微结构未受到显著伤害, 但其一些抵御寒冷的适应性能力有变化。质膜呈波浪状, 或质壁分离, 或凹陷、外凸形成 小泡; 中央大液泡和细胞质内、以及内质网和高尔基体附近多处出现泡状结构或小泡; 多数质体变形为哑铃形、变形虫形、 马蹄形、镰刀形和棒状等, 其中的淀粉粒显著减少。

关键词：豌豆; 低温; 下胚轴细胞; 超微结构

收稿：2009-08-20   修定：2009-09-18

资助：浙江省教育厅科研项目(20060385)。

Adapted Responses of Hypocotylar Cell Ultrastructure in Garden Pea (Pisum sativum Linn.) at Low Temperature

CHEN Xu-Wei*

College of Teacher Education, Wenzhou University, Wenzhou, Zhejiang 325035, China

Corresponding author: CHEN Xu-Wei; E-mail: chenxuwei@126.com; Tel: 0577-86680941

Abstract:

Responses of hypocotylar cell ultrastructure in garden pea (Pisum sativum Linn.) were investigated after 2-day stress at 10 ℃ by electronic transmission microscope. The results showed that hypocotylar cells did not suffer a significant damage at 10 ℃; instead, low temperature could induce some adapted responses. For example, plasma membrane became undulated, plasmolysis was observed, and small vacuoles were formed by invagination or evagination. Small vacuoles appeared in central vavuole or cytoplasm, even nearby endoplasmic reticulum or Golgi apparatus. The form of plastids became dumb-bell shaped, amoeba-shaped, horseshoeshaped, sickle-shaped or rod-like, and starch grains in plastids reduced obviously.

Key words: garden pea (Pisum sativum Linn.); low temperature; hypocotylar cell; ultrastructure